Journal: Journal of orthopaedic surgery (Hong Kong)

Article Title: Naringenin alleviates bone cancer pain via NF-κB/uPA/PAR2 pathway in mice.

doi: 10.1177/10225536241266671

Figure Lengend Snippet: Figure 4. Naringenin inhibited the level of NF-κB/uPA pathway in BCP The level of uPA in serum and bone tissue and the levels of NF-κB pathway-related proteins containing p-IKKβ, IKKβ, p-p65 and p65 were determined in this part. (a), the level of uPA in serum measured by ELISA. (b), the level of uPA in bone tissue measured by ELISA. (c), the levels of p-IKKβ, IKKβ, p-p65 and p65 using western blot. (d), the levels of uPA and p-p65 using immunocytochemistry. The data are presented as the mean ± standard deviation. n = 8. *p < .05, **p < .01 and ***p < .001.

Article Snippet: Then, membrane was blocked by blocking buffer (Beyotime, China) at 4°C for 4 h, followed by the incubationwith the primary antibodies of TNFα (ab183218, 1:1000), TNFR1 (Abcam, ab223352, 1:1000), TNFRSF1A Associated Via Death Domain (TRADD) (Abcam, ab110644, 1:1000), RIP (Abcam, ab202985, 1:1000), TNF Receptor Associated Factor 2 (TRAF2) (Abcam, ab244317, 0.4 μg/mL), p-Inhibitor of Nuclear Factor Kappa B Kinase Subunit Beta (p-IKKβ) (Cell Signaling, #2697, 1:1000), IKKβ (Cell Signaling, #8943, 1:1000), p-p65 (Cell Signaling, #3033, 1:1000), p65 (Cell Signaling, #8242, 1:1000), PAR2 (Abcam, ab180953, 1:10,000), PKC-γ (Abcam, ab71558, 1: 2000), PKA (Cell Signaling, #4782, 1:1000) and TRPV1 (Abcam, ab6166, 1:1000) for 12 h at 4°C.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Standard Deviation

Journal: Plants

Article Title: A Comprehensive Review on Cannabis sativa Ethnobotany, Phytochemistry, Molecular Docking and Biological Activities

doi: 10.3390/plants12061245

Figure Lengend Snippet: Molecular docking of Cannabis sativa L. compounds.

Article Snippet: Inhibitor of nuclear factor kappa-b kinase subunit β (IKKbeta) , 3BRT (p) , Inhibitor kappa kinase , Anti-inflammatory , CBD , Cannabinoids , AutoDock-tools , −7.99 , , [ ] .

Techniques: Activity Assay